IVIS Spectrum

The in vivo Imaging System (IVIS) spectrum is ideally suited for fast imaging (5 animals simultaneaously) of fluorescent or bioluminescent signals for both in vivo as ex vivo. The system enables spectral unmixing for co-registering multiple fluorescent or luminescent signals and can perform 2D or 3D analysis. It provided quantitative data on the fluorescent or luminescent output.

Typical applications of this system include:

Oncology (tumor growth, metabolism, blood vessel leakiness,targeted therapy)
Immunology (localization of different cell populations)
Infectious diseases (fungal growth, viral transmission, therapeutic efficacy)

An overview of some of the possibilities of the IVIS Spectrum for non-invasive optical imaging in small rodent models can be found below.

GFP expressing tumor cells

Spextral unmixing of tissue and GFP

Enlarged image

Luminescent tumor cells in 3D

Fluorescent nanoparticles in 3D

Fluorescent nanoparticles targeting a subcutaneous tumor, visualized by both BLI (tumor cell presence, see figure 2) and fluorescence (nanoparticle presence)

renca 3D pilot

3D image of luminescent cancer cells homing towards the lungs

blood flow

protein accumulation1

Accumulation of fluorescently tagged protein into solid tumor, time point 1 (1 min post injection)

protein accumulation 2

Accumulation of fluorescently tagged protein into solid tumor, time point 2 (15 min post injection)

protein accumulation 3

Accumulation of fluorescently tagged protein into solid tumor, time point 3 (45 min post injection)

protein accumulation 4

Accumulation of fluorescently tagged protein into solid tumor, time point 4 (60 min post injection)